With crisp resolution to 100 nanometers -- a typical germ is about 1,000 nanometers -- the DeltaVision OMX imaging system is considered one of the world's finest microscope systems. Upon its arrival to Indiana University Bloomington's Light Microscopy Imaging Center in 2010, researchers quickly renamed it the "OMG" microscope for the amazing images it produced and for its ability to do super-speed imaging of multiple-labeled proteins in cells.
Today one of those images -- a stunning image taken by longtime IU research associate Jane Stout of a dividing mammalian cell with chromosomes shown aligned on cell division machinery at the sites of attachment -- was announced as a winner in the international GE Healthcare Life Sciences 2012 Cell Imaging Competition.
Stout conducts research in the laboratory of Claire Walczak, a professor of biochemistry and molecular biology in IU Bloomington's Medical Sciences Program, a branch of the IU School of Medicine. Walczak is also executive director of the Light Microscopy Imaging Center in Myers Hall, where the $1.2 million OMX microscope system resides. IU purchased the super-resolution microscope with funds provided solely through the American Recovery and Reinvestment Act of 2009.
"Some of us affectionately renamed it 'OMG' after we saw the images it could produce," Stout recalled. "This instrument, one of only a handful in the world, allows us to see details inside the cells at previously unprecedented resolution."
Stout acknowledged that the initial investment for the microscope was high, but the methodology to use the scope is relatively fast, cheap and highly reproducible, all of which allows researchers to process and image many cells and to look at many different players in this process. The microscope even allows scientists to watch mitosis -- the process of chromosome separation into two identical sets -- in living cells using fluorescently tagged proteins.
Walczak's lab, whose work is funded by the National Institutes of Health, is interested in a specific family of proteins involved in attaching and moving DNA along a subcellular structure called a spindle that separates chromosomes during cell division. The protein family also helps to rearrange the spindle structure itself, and both of these processes occur countless times within humans throughout life, all with an astonishingly high degree of accuracy and precision.
"This particular high-resolution image allowed us to see individual strands within bundles of specialized structures that form the spindle, whereas before we could only infer the bundled structure from other types of imaging and assays," Stout said. "In future images, we hope to see where the different members of the protein family act on the spindle to learn how their movements are coordinated to regulate the entire process of DNA segregation."